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Biological Staining Agent Basic Violet 14
Basic Dyes

Biological Staining Agent Basic Violet 14

1. Product Name: Basic Fuchsin, Rosaniline, Basic Magenta, Basic Violet 14

2. CAS No. 632-99-5. Color Index No. C.I. 42510

3. Molecular Formula: C₂₀H₂₀ClN₃, Molecular Weight: 337.85 g/mol

4. Appearance: Basic Violet 14 appears as a dark green crystalline or lumpy powder with a metallic luster.

5. Solubility: Slightly soluble in water, the solution is reddish-purple; very soluble in alcohol (ethanol), the solution is red.

    Introduction

    Basic Violet 14 (Basic Magenta) is a deep green powdery dye widely used in industry, research, and laboratories. Its most common name is Basic Fuchsin, and it belongs to the triphenylmethane group of dyes.

    Physicochemical Properties

    Melting and Boiling Points
    Melting point approximately 250°C, boiling point approximately 589.3°C.
    pH Indicator
    Within the pH range of 1.0-3.1, the solution color changes from purple to red, thus serving as an acid-base indicator.
    Chemical Properties

    Turns yellowish-brown with concentrated sulfuric acid, becoming almost colorless upon dilution.

    Aqueous solution reacts with sodium hydroxide to form a red precipitate.

    Color Characteristics

    Aqueous solution is reddish-purple; ethanol solution is red.

    Maximum Absorption Wavelength (in ethanol): 543 nm.

    Main Applications

    Due to its unique chemical properties, Basic Violet 14 (Basic Fuchsin) has the following main applications:

    Biological Staining Agent The strongest nuclear dye, capable of clearly staining cell nuclei, mycoplasma, and elastic tissues. It is a key staining agent for identifying Mycobacterium tuberculosis in bacteriology.
    Analytical Chemistry Used in the preparation of Schiff's reagent, a standard reagent specifically for detecting aldehydes. Also used for the detection of certain metal ions (such as Au³⁺).
    Industrial Dyes Can be used for dyeing cotton, synthetic fibers, silk, paper, and leather, as well as for coloring inks and paints.
    Emerging Research Studies have shown its potential applications in electronic devices such as organic semiconductors.

    Safety and Handling

    Basic Violet 14 is a hazardous chemical; strict adherence to safety regulations is required during handling.

    Health Hazards:

    Harmful if ingested.
    May cause skin, eye, and respiratory irritation, leading to allergic reactions such as rash, tearing, and coughing.
    Excessive exposure or inhalation may cause dizziness, nausea, muscle weakness, and even respiratory failure.
    Long-term or repeated exposure carries a carcinogenic risk, with particular attention to bladder cancer.

    Environmental Impact:

    Extremely toxic to aquatic life and may produce long-term, persistent harmful effects.

    Storage and Handling

    Storage: Store in a sealed, cool, and dry place. Some data recommend refrigeration.

    Spill Handling: First, wet the solid spill with water, then transfer to a suitable container and thoroughly clean the contaminated surface.

    Fire Extinguishing: Dry powder, carbon dioxide, or halon fire extinguishing agents are recommended.

    In summary, Basic Violet 14 (Basic Magenta) is a widely used chemical but carries certain health and environmental risks. If you are using it in a laboratory or industrial environment, please always consult the latest Material Safety Data Sheet (MSDS) for detailed safe handling guidelines.

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